H2N2V1, Main, Exploration, bibRecord, 000C97

A Reassortment-Incompetent Live Attenuated Influenza Virus Vaccine for Protection against Pandemic Virus Strains▿

Identifieur interne : 000C97 ( Main/Exploration ); précédent : 000C96; suivant : 000C98

A Reassortment-Incompetent Live Attenuated Influenza Virus Vaccine for Protection against Pandemic Virus Strains▿

Auteurs : Rong Hai ; Adolfo García-Sastre [États-Unis] ; David E. Swayne [États-Unis] ; Peter Palese [États-Unis]

Source :

Journal of Virology [ 0022-538X ] ; 2011.

RBID : PMC:3126605

Abstract

Although live-attenuated influenza vaccines (LAIV) are safe for use in protection against seasonal influenza strains, concerns regarding their potential to reassort with wild-type virus strains have been voiced. LAIVs have been demonstrated to induce enhanced mucosal and cell-mediated immunity better than inactivated vaccines while also requiring a smaller dose to achieve a protective immune response. To address the need for a reassortment-incompetent live influenza A virus vaccine, we have designed a chimeric virus that takes advantage of the fact that influenza A and B viruses do not reassort. Our novel vaccine prototype uses an attenuated influenza B virus that has been manipulated to express the ectodomain of the influenza A hemagglutinin protein, the major target for eliciting neutralizing antibodies. The hemagglutinin RNA segment is modified such that it contains influenza B packaging signals, and therefore it cannot be incorporated into a wild-type influenza A virus. We have applied our strategy to different influenza A virus subtypes and generated chimeric B/PR8 HA (H1), HK68 (H3), and VN (H5) viruses. All recombinant viruses were attenuated both in vitro and in vivo, and immunization with these recombinant viruses protected mice against lethal influenza A virus infection. Overall, our data indicate that the chimeric live-attenuated influenza B viruses expressing the modified influenza A hemagglutinin are effective LAIVs.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126605

DOI: 10.1128/JVI.00609-11
PubMed: 21543486
PubMed Central: 3126605

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Reassortment-Incompetent Live Attenuated Influenza Virus Vaccine for Protection against Pandemic Virus Strains<xref ref-type="fn" rid="FN1">▿</xref>
</title>
<author><name sortKey="Hai, Rong" sort="Hai, Rong" uniqKey="Hai R" first="Rong" last="Hai">Rong Hai</name>
<affiliation><nlm:aff id="aff1">Department of Microbiology</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Garcia Sastre, Adolfo" sort="Garcia Sastre, Adolfo" uniqKey="Garcia Sastre A" first="Adolfo" last="García-Sastre">Adolfo García-Sastre</name>
<affiliation><nlm:aff id="aff1">Department of Microbiology</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Institute of Global Health and Emerging Pathogens</nlm:aff>
</affiliation>
<affiliation wicri:level="2"><nlm:aff id="aff3">Department of Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, New York</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine, New York</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Swayne, David E" sort="Swayne, David E" uniqKey="Swayne D" first="David E." last="Swayne">David E. Swayne</name>
<affiliation wicri:level="2"><nlm:aff id="aff4">USDA-ARS, Southeast Poultry Research Laboratory, Exotic and Emerging Avian Viral Diseases Research Unit, Athens, Georgia 30605-2195</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>USDA-ARS, Southeast Poultry Research Laboratory, Exotic and Emerging Avian Viral Diseases Research Unit, Athens</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Palese, Peter" sort="Palese, Peter" uniqKey="Palese P" first="Peter" last="Palese">Peter Palese</name>
<affiliation><nlm:aff id="aff1">Department of Microbiology</nlm:aff>
</affiliation>
<affiliation wicri:level="2"><nlm:aff id="aff3">Department of Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, New York</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine, New York</wicri:cityArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">21543486</idno>
<idno type="pmc">3126605</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126605</idno>
<idno type="RBID">PMC:3126605</idno>
<idno type="doi">10.1128/JVI.00609-11</idno>
<date when="2011">2011</date>
<idno type="wicri:Area/Pmc/Corpus">000636</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000636</idno>
<idno type="wicri:Area/Pmc/Curation">000636</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000636</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000860</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000860</idno>
<idno type="wicri:Area/Ncbi/Merge">000646</idno>
<idno type="wicri:Area/Ncbi/Curation">000646</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000646</idno>
<idno type="wicri:doubleKey">0022-538X:2011:Hai R:a:reassortment:incompetent</idno>
<idno type="wicri:Area/Main/Merge">000D02</idno>
<idno type="wicri:Area/Main/Curation">000C97</idno>
<idno type="wicri:Area/Main/Exploration">000C97</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Reassortment-Incompetent Live Attenuated Influenza Virus Vaccine for Protection against Pandemic Virus Strains<xref ref-type="fn" rid="FN1">▿</xref>
</title>
<author><name sortKey="Hai, Rong" sort="Hai, Rong" uniqKey="Hai R" first="Rong" last="Hai">Rong Hai</name>
<affiliation><nlm:aff id="aff1">Department of Microbiology</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Garcia Sastre, Adolfo" sort="Garcia Sastre, Adolfo" uniqKey="Garcia Sastre A" first="Adolfo" last="García-Sastre">Adolfo García-Sastre</name>
<affiliation><nlm:aff id="aff1">Department of Microbiology</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Institute of Global Health and Emerging Pathogens</nlm:aff>
</affiliation>
<affiliation wicri:level="2"><nlm:aff id="aff3">Department of Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, New York</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine, New York</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Swayne, David E" sort="Swayne, David E" uniqKey="Swayne D" first="David E." last="Swayne">David E. Swayne</name>
<affiliation wicri:level="2"><nlm:aff id="aff4">USDA-ARS, Southeast Poultry Research Laboratory, Exotic and Emerging Avian Viral Diseases Research Unit, Athens, Georgia 30605-2195</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>USDA-ARS, Southeast Poultry Research Laboratory, Exotic and Emerging Avian Viral Diseases Research Unit, Athens</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Palese, Peter" sort="Palese, Peter" uniqKey="Palese P" first="Peter" last="Palese">Peter Palese</name>
<affiliation><nlm:aff id="aff1">Department of Microbiology</nlm:aff>
</affiliation>
<affiliation wicri:level="2"><nlm:aff id="aff3">Department of Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine, New York, New York</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Department of Medicine, Division of Infectious Diseases, Mount Sinai School of Medicine, New York</wicri:cityArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2011">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Although live-attenuated influenza vaccines (LAIV) are safe for use in protection against seasonal influenza strains, concerns regarding their potential to reassort with wild-type virus strains have been voiced. LAIVs have been demonstrated to induce enhanced mucosal and cell-mediated immunity better than inactivated vaccines while also requiring a smaller dose to achieve a protective immune response. To address the need for a reassortment-incompetent live influenza A virus vaccine, we have designed a chimeric virus that takes advantage of the fact that influenza A and B viruses do not reassort. Our novel vaccine prototype uses an attenuated influenza B virus that has been manipulated to express the ectodomain of the influenza A hemagglutinin protein, the major target for eliciting neutralizing antibodies. The hemagglutinin RNA segment is modified such that it contains influenza B packaging signals, and therefore it cannot be incorporated into a wild-type influenza A virus. We have applied our strategy to different influenza A virus subtypes and generated chimeric B/PR8 HA (H1), HK68 (H3), and VN (H5) viruses. All recombinant viruses were attenuated both <italic>in vitro</italic>
 and <italic>in vivo</italic>
, and immunization with these recombinant viruses protected mice against lethal influenza A virus infection. Overall, our data indicate that the chimeric live-attenuated influenza B viruses expressing the modified influenza A hemagglutinin are effective LAIVs.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Géorgie (États-Unis)</li>
<li>État de New York</li>
</region>
</list>
<tree><noCountry><name sortKey="Hai, Rong" sort="Hai, Rong" uniqKey="Hai R" first="Rong" last="Hai">Rong Hai</name>
</noCountry>
<country name="États-Unis"><region name="État de New York"><name sortKey="Garcia Sastre, Adolfo" sort="Garcia Sastre, Adolfo" uniqKey="Garcia Sastre A" first="Adolfo" last="García-Sastre">Adolfo García-Sastre</name>
</region>
<name sortKey="Palese, Peter" sort="Palese, Peter" uniqKey="Palese P" first="Peter" last="Palese">Peter Palese</name>
<name sortKey="Swayne, David E" sort="Swayne, David E" uniqKey="Swayne D" first="David E." last="Swayne">David E. Swayne</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C97 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000C97 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    H2N2V1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:3126605
   |texte=   A Reassortment-Incompetent Live Attenuated Influenza Virus Vaccine for Protection against Pandemic Virus Strains▿
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:21543486" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a H2N2V1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 14 19:59:40 2020. Site generation: Thu Mar 25 15:38:26 2021

Serveur d'exploration H2N2

A Reassortment-Incompetent Live Attenuated Influenza Virus Vaccine for Protection against Pandemic Virus Strains▿

A Reassortment-Incompetent Live Attenuated Influenza Virus Vaccine for Protection against Pandemic Virus Strains▿

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration H2N2
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.